Miguel Alcalde Group

Thesis

PhD Thesis

Directed evolution of unspecific peroxygenase: Synthesis of human drug metabolites and design of functional fusion enzymes

Student: Patricia Gomez de Santos
University: Universidad Autónoma de Madrid
Date: 16/12/2020

Evolución dirigida de la peroxigenasa inespecífica de Agrocybe aegerita: Tolerancia a disolventes orgánicos mediante deriva genética neutral y evolución adaptativa

Student: Javier Martín Díaz
University: Universidad Autónoma de Madrid
Date: 24/07/2019

Diseño de la peroxigenasa inespecífica de Agrocybe aegerita mediante evolución dirigida: expresión funcional en levaduras

Student: Patricia Molina Espeja
University: Universidad Autónoma de Madrid
Date: 31/3/2016

Evolución Dirigida de peroxidasa versátil de Pleurotus eryngii en Saccharomyces cerevisiae

Student: Eva García Ruiz
University: Universidad Complutense de Madrid
Date: 15/11/2012

Directed evolution of ancestral and modern enzymes

Student: Bernardo Gomez Fernandez
University: Universidad Autónoma de Madrid
Date: 18/10/2019

Directed and computational evolution of fungal laccases: redox potential enhancement and development of a family of thermostable chimeras

Student: Ivan Mateljak
University: Universidad Autónoma de Madrid
Date:18/10/2017

Evolución dirigida de la peroxidasa versátil para el diseño de una levadura de podredumbre blanca

Student: David González Pérez
University: Universidad Autónoma de Madrid
Date: 16/12/2016

Evolución molecular dirigida de lacasas fúngicas en Saccharomyces cerevisiae: tolerancia a disolventes orgánicos

Student: Miren Zumárraga Uribesalgo
University: Universidad Autónoma de Madrid
Date: September 2007

Biotechnology platforms for aryl-alcohol oxidases by directed evolution

Student: Javier Viña Gonzalez
University: Universidad Autónoma de Madrid
Date: 03/10/2019

Evolución dirigida de lacasas fúngicas: termoestabilidad y síntesis de colorantes heteropoliméricos

Student: Ana Isabel Vicente Martín
University: Universidad Complutense de Madrid
Date: 09/03/2017

Diseño de lacasas fúngicas activas en sangre mediante evolución molecular dirigida

Student: Diana Maté Mate
University: Universidad Autónoma de Madrid
Date: 25/06/2013

Síntesis enzimática de oligosacáridos de carácter  prebiótico

Student: Arantza Gómez de Segura Ugalde
University: Universidad Autónoma de Madrid
Date: June 2004

Directed evolution of unspecific peroxygenase: Synthesis of human drug metabolites and design of functional fusion enzymes

Student: Patricia Gomez de Santos

University: Universidad Autónoma de Madrid
Date: 16/12/2020

Directed evolution of ancestral and modern enzymes

Student: Bernardo Gomez Fernandez

University: Universidad Autónoma de Madrid
Date: 18/10/2019

Biotechnology platforms for aryl-alcohol oxidases by directed evolution

Student: Javier Viña Gonzalez

University: Universidad Autónoma de Madrid
Date:03/10/2019

Evolución dirigida de la peroxigenasa inespecífica de Agrocybe aegerita: Tolerancia a disolventes orgánicos mediante deriva genética neutral y evolución adaptativa

Student: Javier Martín Díaz

University: Universidad Autónoma de Madrid
Date:24/07/2019

Directed and ccomputational evolution of fungal laccases: redox potential enhancement and development of a family of thermostable chimeras

Student: Ivan Mateljak

University: Universidad Autónoma de Madrid
Date:18/10/2017

Evolución dirigida de lacasas fúngicas: termoestabilidad y síntesis de colorantes heteropoliméricos

Student: Ana Isabel Vicente Martín
University: Universidad Complutense de Madrid
Date: 09/03/2017

Evolución dirigida de la peroxidasa versátil para el diseño de una levadura de podredumbre blanca

Student: David González Pérez
University: Universidad Autónoma de Madrid
Date: 16/12/2016

Diseño de la peroxigenasa inespecífica de Agrocybe aegerita mediante evolución dirigida: expresión funcional en levaduras

Student: Patricia Molina Espeja
University: Universidad Autónoma de Madrid
Date: 31/3/2016

Diseño de lacasas fúngicas activas en sangre mediante evolución molecular dirigida

Student: Diana Maté Mate
University: Universidad Autónoma de Madrid
Date: 25/06/2013

Evolución Dirigida de peroxidasa versátil de Pleurotus eryngii en Saccharomyces cerevisiae

Student: Eva García Ruiz
University: Universidad Complutense de Madrid
Date: 15/11/2012

Evolución molecular dirigida de lacasas fúngicas en Saccharomyces cerevisiae: tolerancia a disolventes orgánicos

Student: Miren Zumárraga Uribesalgo
University: Universidad Autónoma de Madrid
Date: September 2007

Síntesis enzimática de oligosacáridos de carácter  prebiótico

Student: Arantza Gómez de Segura Ugalde
University: Universidad Autónoma de Madrid
Date: June 2004

Master Thesis

Engineering thermostable cutinases bySCHEMA-RASPP recombination and ancestral reconstruction for controlled bioplastic degradation

Student: Julia Murciano
University: Universidad Autónoma de Madrid
Date: June 2024

Urate oxidases directed evolution platform for the development of a continuous biosensor of uric acid

Student: Carolina María Rodríguez
University: Universidad Europea de Madrid
Date: June 2024

Peroxygenase engineering for studies on degradation of complex epoxy materials

Student: Sara Andrés Campos
University: Universidad Europea de Madrid
Date: May 2022

Expresión heteróloga de proteínas de fusión en Escherichia coli para explorar la diversidad natural de peroxigenasas fúngicas

Student: Marta Del Pozo Medina
University: Universidad Complutense de Madrid
Date: July 2021

Evolución molecular dirigida de la aril-alcohol oxidasa de Pleurotus eryngii para la oxidación de alcoholes acrílicos secundarios

Student: Diego Esteban Lalana
University: Universidad Complutense de Madrid
Date: July 2018

Design of chimeric fusions of evolved Aryl alcohol oxidase-Unspecific peroxygenase in Saccharomyces cerevisiae

Student: Sofia Lazaro
University: Universidad Complutense de Madrid
Date: February 2018

Laboratory evolution of unspecific peroxygenase for activity in organic solvents

Student: Solymar J. del Pino
University: Universidad Autónoma de Madrid
Date: September 2016

Directed evolution of aryl-alcohol oxidase using a tandem-yeast expression system

Student: Katarina Elbl
University: Faculty Applied Chemistry, Germany.
Date: January 2016

Engineering oxidative stability in versatile peroxidase by directed evolution

Student: Alina Ioana Roman
University: Universidad Autónoma de Madrid
Date: June 2011

Expresión funcional de peroxidasas de alto potencial redox mediante Evolución Dirigida

Student: Eva García Ruiz
University: Universidad Complutense de Madrid
Date: September 2009

Evolución molecular dirigida de lacasas de alto potencial redox

Student: Diana Maté Mate
University: Universidad Autónoma de Madrid
Date: June 2009

Thermostable cutinases for polyester resins degradation by directed evolution

Student: Nuria Velasco
University: Universidad Autónoma de Madrid
Date: June 2024

Characterization of a computational library of an ancestral hydrolase

Student: Maxim van Delft
University: TuDelft
Date: June 2023

Exploring the functional heterologous expression of unspecific peroxygenases in Escherichia coli by designing SUMO-UPO fusions

Student: Andrea Menés Rubio
University: Universidad Autónoma de Madrid
Date: June 2021

Unspecific peroxygenase engineering: analysis of structural and catalytic residues

Student: Maria Alejandra Alfuzzi
University: Universidad Autónoma de Madrid
Date: June 2018

Directed evolution of a manganese peroxidase from Ceriporiopsis subvermispora towards functional expression

Student: Berndjan Eenink
University: Wageningen University, The Netherlands
Date: October 2016

Directed-focused-evolution of unspecific peroxygenase: improving thermostability and peroxygenase activity

Student: Miguel Arcángel Palomino
University: Universidad Autónoma de Madrid
Date: September 2016

Shuffling the neutral drift of unspecific peroxygenase expressed in yeast

Student: Carmen Paret Fernández
University: Universidad Autónoma de Madrid
Date: July 2015

Chimeric signal peptides for functional expression of aryl alcohol oxidase in Saccharomyces cerevisiae

Student: Javier Viña González
University: Universidad Autónoma de Madrid
Date: July 2013

Engineering chimeric laccases by directed evolution

 

Student: Isabel Pardo
University: Universidad Autónoma de Madrid
Date: September 2011

Evolución molecular dirigida de la lacasa de alto potencial redox del hongo Pycnoporus cinnabarinus

Student: Patricia Molina Espeja
University: Universidad Autónoma de Madrid
Date: September 2009

Evolución dirigida de lacasas para su actividad en disolventes orgánicos

Student: Miren Zumárraga Uribesalgo
University: Universidad Autónoma de Madrid
Date: October 2005

Engineering thermostable cutinases bySCHEMA-RASPP recombination and ancestral reconstruction for controlled bioplastic degradation

Student: Julia Murciano
University: Universidad Autónoma de Madrid
Date: June 2024

Thermostable cutinases for polyester resins degradation by directed evolution

Student: Nuria Velasco
University: Universidad Autónoma de Madrid
Date: June 2024

Urate oxidases directed evolution platform for the development of a continuous biosensor of uric acid

Student: Carolina María Rodríguez
University: Universidad Europea de Madrid
Date: June 2024

Characterization of a computational library of an ancestral hydrolase

Student: Maxim van Delft
University: TuDelft
Date: June 2023

Reconstruction and resurrection of short type ancestral peroxygenases for biotechnological purposes

Student: Pablo Velazquez García
University: Universidad Autónoma de Madrid
Date: July 2022

Peroxygenase engineering for studies on degradation of complex epoxy materials

Student: Sara Andrés Campos
University: Universidad Europea de Madrid
Date: May 2022

Expresión heteróloga de proteínas de fusión en Escherichia coli para explorar la diversidad natural de peroxigenasas fúngicas

Student: Marta Del Pozo Medina
University: Universidad Complutense de Madrid
Date: July 2021

Exploring the functional heterologous expression of unspecific peroxygenases in Escherichia coli by designing SUMO-UPO fusions

Student: Andrea Menés Rubio
University: Universidad Autónoma de Madrid
Date: June 2021

Evolución molecular dirigida de la aril-alcohol oxidas de Pleurotus eryngii para la oxidación de alcoholes acrílicos secundarios

Student: Diego Esteban Lalana
University: Universidad Complutense de Madrid
Date: July 2018

Unspecific peroxygenase engineering: analysis of structural and catalytic residues

Student: Alejandra Alfuzzi
University: Universidad Autónoma de Madrid
Date: June 2018

Design of chimeric fusions of evolved Aryl alcohol oxidase-Unspecific peroxygenase in Saccharomyces cerevisiae

Student: Sofia Lazaro
University: Universidad Complutense de Madrid
Date: February 2018

Directed evolution of a manganese peroxidase from Ceriporiopsis subvermispora towards functional expression

Student: Berndjan Eenink
University: Wageningen University, The Netherlands
Date: October 2016

Directed-focused-evolution of unspecific peroxygenase: improving thermostability and peroxygenase activity

Student: Miguel Arcángel Palomino
University: Universidad Autónoma de Madrid
Date: September 2016

Laboratory evolution of unspecific peroxygenase for activity in organic solvents

Student: Solymar J. del Pino
University: Universidad Autónoma de Madrid
Date: September 2016

Directed evolution of aryl-alcohol oxidase using a tandem-yeast expression system

Student: Katarina Elbl
University: Faculty Applied Chemistry, Germany.
Date: January 2016

Shuffling the neutral drift of unspecific peroxygenase expressed in yeast

Student: Carmen Paret Fernández
University: Universidad Autónoma de Madrid
Date: July 2015

Chimeric signal peptides for functional expression of aryl alcohol oxidase in Saccharomyces cerevisiae

Student: Javier Viña González
University: Universidad Autónoma de Madrid
Date: July 2013

Engineering chimeric laccases by directed evolution

 

Student: Isabel Pardo
University: Universidad Autónoma de Madrid
Date: September 2011

Engineering oxidative stability in versatile peroxidase by directed evolution

Student: Alina Ioana Roman
University: Universidad Autónoma de Madrid
Date: June 2011

Expresión funcional de peroxidasas de alto potencial redox mediante Evolución Dirigida

Student: Eva García Ruiz
University: Universidad Complutense de Madrid
Date: September 2009

Evolución molecular dirigida de la lacasa de alto potencial redox del hongo Pycnoporus cinnabarinus

Student: Patricia Molina Espeja
University: Universidad Autónoma de Madrid
Date: September 2009

Evolución molecular dirigida de lacasas de alto potencial redox

Student: Diana Maté Mate
University: Universidad Autónoma de Madrid
Date: June 2009

Evolución dirigida de lacasas para su actividad en disolventes orgánicos

Student: Miren Zumárraga Uribesalgo
University: Universidad Autónoma de Madrid
Date: October 2005

Miguel Alcalde Laboratory
Institute of Catalysis, ICP-CSIC,
Cantoblanco, 28049, Madrid, Spain
Tel: +34 915854806
Fax: +34 915854760