PhD Thesis
Directed evolution of unspecific peroxygenase: Synthesis of human drug metabolites and design of functional fusion enzymes
Student: Patricia Gomez de Santos
University: Universidad Autónoma de Madrid
Date: 16/12/2020
Evolución dirigida de la peroxigenasa inespecífica de Agrocybe aegerita: Tolerancia a disolventes orgánicos mediante deriva genética neutral y evolución adaptativa
Student: Javier Martín Díaz
University: Universidad Autónoma de Madrid
Date: 24/07/2019
Diseño de la peroxigenasa inespecífica de Agrocybe aegerita mediante evolución dirigida: expresión funcional en levaduras
Student: Patricia Molina Espeja
University: Universidad Autónoma de Madrid
Date: 31/3/2016
Evolución Dirigida de peroxidasa versátil de Pleurotus eryngii en Saccharomyces cerevisiae
Student: Eva García Ruiz
University: Universidad Complutense de Madrid
Date: 15/11/2012
Directed evolution of ancestral and modern enzymes
Student: Bernardo Gomez Fernandez
University: Universidad Autónoma de Madrid
Date: 18/10/2019
Directed and computational evolution of fungal laccases: redox potential enhancement and development of a family of thermostable chimeras
Student: Ivan Mateljak
University: Universidad Autónoma de Madrid
Date:18/10/2017
Evolución dirigida de la peroxidasa versátil para el diseño de una levadura de podredumbre blanca
Student: David González Pérez
University: Universidad Autónoma de Madrid
Date: 16/12/2016
Evolución molecular dirigida de lacasas fúngicas en Saccharomyces cerevisiae: tolerancia a disolventes orgánicos
Student: Miren Zumárraga Uribesalgo
University: Universidad Autónoma de Madrid
Date: September 2007
Biotechnology platforms for aryl-alcohol oxidases by directed evolution
Student: Javier Viña Gonzalez
University: Universidad Autónoma de Madrid
Date: 03/10/2019
Evolución dirigida de lacasas fúngicas: termoestabilidad y síntesis de colorantes heteropoliméricos
Student: Ana Isabel Vicente Martín
University: Universidad Complutense de Madrid
Date: 09/03/2017
Diseño de lacasas fúngicas activas en sangre mediante evolución molecular dirigida
Student: Diana Maté Mate
University: Universidad Autónoma de Madrid
Date: 25/06/2013
Síntesis enzimática de oligosacáridos de carácter prebiótico
Student: Arantza Gómez de Segura Ugalde
University: Universidad Autónoma de Madrid
Date: June 2004
Directed evolution of unspecific peroxygenase: Synthesis of human drug metabolites and design of functional fusion enzymes
Student: Patricia Gomez de Santos
University: Universidad Autónoma de Madrid
Date: 16/12/2020
Directed evolution of ancestral and modern enzymes
Student: Bernardo Gomez Fernandez
University: Universidad Autónoma de Madrid
Date: 18/10/2019
Biotechnology platforms for aryl-alcohol oxidases by directed evolution
Student: Javier Viña Gonzalez
University: Universidad Autónoma de Madrid
Date:03/10/2019
Evolución dirigida de la peroxigenasa inespecífica de Agrocybe aegerita: Tolerancia a disolventes orgánicos mediante deriva genética neutral y evolución adaptativa
Student: Javier Martín Díaz
University: Universidad Autónoma de Madrid
Date:24/07/2019
Directed and ccomputational evolution of fungal laccases: redox potential enhancement and development of a family of thermostable chimeras
Student: Ivan Mateljak
University: Universidad Autónoma de Madrid
Date:18/10/2017
Evolución dirigida de lacasas fúngicas: termoestabilidad y síntesis de colorantes heteropoliméricos
Student: Ana Isabel Vicente Martín
University: Universidad Complutense de Madrid
Date: 09/03/2017
Evolución dirigida de la peroxidasa versátil para el diseño de una levadura de podredumbre blanca
Student: David González Pérez
University: Universidad Autónoma de Madrid
Date: 16/12/2016
Diseño de la peroxigenasa inespecífica de Agrocybe aegerita mediante evolución dirigida: expresión funcional en levaduras
Student: Patricia Molina Espeja
University: Universidad Autónoma de Madrid
Date: 31/3/2016
Diseño de lacasas fúngicas activas en sangre mediante evolución molecular dirigida
Student: Diana Maté Mate
University: Universidad Autónoma de Madrid
Date: 25/06/2013
Evolución Dirigida de peroxidasa versátil de Pleurotus eryngii en Saccharomyces cerevisiae
Student: Eva García Ruiz
University: Universidad Complutense de Madrid
Date: 15/11/2012
Evolución molecular dirigida de lacasas fúngicas en Saccharomyces cerevisiae: tolerancia a disolventes orgánicos
Student: Miren Zumárraga Uribesalgo
University: Universidad Autónoma de Madrid
Date: September 2007
Síntesis enzimática de oligosacáridos de carácter prebiótico
Student: Arantza Gómez de Segura Ugalde
University: Universidad Autónoma de Madrid
Date: June 2004
Master Thesis
Engineering thermostable cutinases bySCHEMA-RASPP recombination and ancestral reconstruction for controlled bioplastic degradation
Student: Julia Murciano
University: Universidad Autónoma de Madrid
Date: June 2024
University: Universidad Autónoma de Madrid
Date: June 2024
Urate oxidases directed evolution platform for the development of a continuous biosensor of uric acid
Student: Carolina María Rodríguez
University: Universidad Europea de Madrid
Date: June 2024
University: Universidad Europea de Madrid
Date: June 2024
Peroxygenase engineering for studies on degradation of complex epoxy materials
Student: Sara Andrés Campos
University: Universidad Europea de Madrid
Date: May 2022
University: Universidad Europea de Madrid
Date: May 2022
Expresión heteróloga de proteínas de fusión en Escherichia coli para explorar la diversidad natural de peroxigenasas fúngicas
Student: Marta Del Pozo Medina
University: Universidad Complutense de Madrid
Date: July 2021
University: Universidad Complutense de Madrid
Date: July 2021
Evolución molecular dirigida de la aril-alcohol oxidasa de Pleurotus eryngii para la oxidación de alcoholes acrílicos secundarios
Student: Diego Esteban Lalana
University: Universidad Complutense de Madrid
Date: July 2018
University: Universidad Complutense de Madrid
Date: July 2018
Design of chimeric fusions of evolved Aryl alcohol oxidase-Unspecific peroxygenase in Saccharomyces cerevisiae
Student: Sofia Lazaro
University: Universidad Complutense de Madrid
Date: February 2018
University: Universidad Complutense de Madrid
Date: February 2018
Laboratory evolution of unspecific peroxygenase for activity in organic solvents
Student: Solymar J. del Pino
University: Universidad Autónoma de Madrid
Date: September 2016
University: Universidad Autónoma de Madrid
Date: September 2016
Directed evolution of aryl-alcohol oxidase using a tandem-yeast expression system
Student: Katarina Elbl
University: Faculty Applied Chemistry, Germany.
Date: January 2016
University: Faculty Applied Chemistry, Germany.
Date: January 2016
Engineering oxidative stability in versatile peroxidase by directed evolution
Student: Alina Ioana Roman
University: Universidad Autónoma de Madrid
Date: June 2011
Expresión funcional de peroxidasas de alto potencial redox mediante Evolución Dirigida
Student: Eva García Ruiz
University: Universidad Complutense de Madrid
Date: September 2009
Evolución molecular dirigida de lacasas de alto potencial redox
Student: Diana Maté Mate
University: Universidad Autónoma de Madrid
Date: June 2009
Thermostable cutinases for polyester resins degradation by directed evolution
Student: Nuria Velasco
University: Universidad Autónoma de Madrid
Date: June 2024
University: Universidad Autónoma de Madrid
Date: June 2024
Characterization of a computational library of an ancestral hydrolase
Student: Maxim van Delft
University: TuDelft
Date: June 2023
University: TuDelft
Date: June 2023
Exploring the functional heterologous expression of unspecific peroxygenases in Escherichia coli by designing SUMO-UPO fusions
Student: Andrea Menés Rubio
University: Universidad Autónoma de Madrid
Date: June 2021
University: Universidad Autónoma de Madrid
Date: June 2021
Unspecific peroxygenase engineering: analysis of structural and catalytic residues
Student: Maria Alejandra Alfuzzi
University: Universidad Autónoma de Madrid
Date: June 2018
University: Universidad Autónoma de Madrid
Date: June 2018
Directed evolution of a manganese peroxidase from Ceriporiopsis subvermispora towards functional expression
Student: Berndjan Eenink
University: Wageningen University, The Netherlands
Date: October 2016
University: Wageningen University, The Netherlands
Date: October 2016
Directed-focused-evolution of unspecific peroxygenase: improving thermostability and peroxygenase activity
Student: Miguel Arcángel Palomino
University: Universidad Autónoma de Madrid
Date: September 2016
University: Universidad Autónoma de Madrid
Date: September 2016
Shuffling the neutral drift of unspecific peroxygenase expressed in yeast
Student: Carmen Paret Fernández
University: Universidad Autónoma de Madrid
Date: July 2015
Chimeric signal peptides for functional expression of aryl alcohol oxidase in Saccharomyces cerevisiae
Student: Javier Viña González
University: Universidad Autónoma de Madrid
Date: July 2013
Engineering chimeric laccases by directed evolution
Student: Isabel Pardo
University: Universidad Autónoma de Madrid
Date: September 2011
Evolución molecular dirigida de la lacasa de alto potencial redox del hongo Pycnoporus cinnabarinus
Student: Patricia Molina Espeja
University: Universidad Autónoma de Madrid
Date: September 2009
Evolución dirigida de lacasas para su actividad en disolventes orgánicos
Student: Miren Zumárraga Uribesalgo
University: Universidad Autónoma de Madrid
Date: October 2005
Date: October 2005
Engineering thermostable cutinases bySCHEMA-RASPP recombination and ancestral reconstruction for controlled bioplastic degradation
Student: Julia Murciano
University: Universidad Autónoma de Madrid
Date: June 2024
University: Universidad Autónoma de Madrid
Date: June 2024
Thermostable cutinases for polyester resins degradation by directed evolution
Student: Nuria Velasco
University: Universidad Autónoma de Madrid
Date: June 2024
University: Universidad Autónoma de Madrid
Date: June 2024
Urate oxidases directed evolution platform for the development of a continuous biosensor of uric acid
Student: Carolina María Rodríguez
University: Universidad Europea de Madrid
Date: June 2024
University: Universidad Europea de Madrid
Date: June 2024
Characterization of a computational library of an ancestral hydrolase
Student: Maxim van Delft
University: TuDelft
Date: June 2023
University: TuDelft
Date: June 2023
Reconstruction and resurrection of short type ancestral peroxygenases for biotechnological purposes
Student: Pablo Velazquez García
University: Universidad Autónoma de Madrid
Date: July 2022
University: Universidad Autónoma de Madrid
Date: July 2022
Peroxygenase engineering for studies on degradation of complex epoxy materials
Student: Sara Andrés Campos
University: Universidad Europea de Madrid
Date: May 2022
University: Universidad Europea de Madrid
Date: May 2022
Expresión heteróloga de proteínas de fusión en Escherichia coli para explorar la diversidad natural de peroxigenasas fúngicas
Student: Marta Del Pozo Medina
University: Universidad Complutense de Madrid
Date: July 2021
University: Universidad Complutense de Madrid
Date: July 2021
Exploring the functional heterologous expression of unspecific peroxygenases in Escherichia coli by designing SUMO-UPO fusions
Student: Andrea Menés Rubio
University: Universidad Autónoma de Madrid
Date: June 2021
University: Universidad Autónoma de Madrid
Date: June 2021
Evolución molecular dirigida de la aril-alcohol oxidas de Pleurotus eryngii para la oxidación de alcoholes acrílicos secundarios
Student: Diego Esteban Lalana
University: Universidad Complutense de Madrid
Date: July 2018
University: Universidad Complutense de Madrid
Date: July 2018
Unspecific peroxygenase engineering: analysis of structural and catalytic residues
Student: Alejandra Alfuzzi
University: Universidad Autónoma de Madrid
Date: June 2018
University: Universidad Autónoma de Madrid
Date: June 2018
Design of chimeric fusions of evolved Aryl alcohol oxidase-Unspecific peroxygenase in Saccharomyces cerevisiae
Student: Sofia Lazaro
University: Universidad Complutense de Madrid
Date: February 2018
University: Universidad Complutense de Madrid
Date: February 2018
Directed evolution of a manganese peroxidase from Ceriporiopsis subvermispora towards functional expression
Student: Berndjan Eenink
University: Wageningen University, The Netherlands
Date: October 2016
University: Wageningen University, The Netherlands
Date: October 2016
Directed-focused-evolution of unspecific peroxygenase: improving thermostability and peroxygenase activity
Student: Miguel Arcángel Palomino
University: Universidad Autónoma de Madrid
Date: September 2016
University: Universidad Autónoma de Madrid
Date: September 2016
Laboratory evolution of unspecific peroxygenase for activity in organic solvents
Student: Solymar J. del Pino
University: Universidad Autónoma de Madrid
Date: September 2016
University: Universidad Autónoma de Madrid
Date: September 2016
Directed evolution of aryl-alcohol oxidase using a tandem-yeast expression system
Student: Katarina Elbl
University: Faculty Applied Chemistry, Germany.
Date: January 2016
University: Faculty Applied Chemistry, Germany.
Date: January 2016
Shuffling the neutral drift of unspecific peroxygenase expressed in yeast
Student: Carmen Paret Fernández
University: Universidad Autónoma de Madrid
Date: July 2015
Chimeric signal peptides for functional expression of aryl alcohol oxidase in Saccharomyces cerevisiae
Student: Javier Viña González
University: Universidad Autónoma de Madrid
Date: July 2013
Engineering chimeric laccases by directed evolution
Student: Isabel Pardo
University: Universidad Autónoma de Madrid
Date: September 2011
Engineering oxidative stability in versatile peroxidase by directed evolution
Student: Alina Ioana Roman
University: Universidad Autónoma de Madrid
Date: June 2011
Expresión funcional de peroxidasas de alto potencial redox mediante Evolución Dirigida
Student: Eva García Ruiz
University: Universidad Complutense de Madrid
Date: September 2009
Evolución molecular dirigida de la lacasa de alto potencial redox del hongo Pycnoporus cinnabarinus
Student: Patricia Molina Espeja
University: Universidad Autónoma de Madrid
Date: September 2009
Evolución molecular dirigida de lacasas de alto potencial redox
Student: Diana Maté Mate
University: Universidad Autónoma de Madrid
Date: June 2009
Evolución dirigida de lacasas para su actividad en disolventes orgánicos
Student: Miren Zumárraga Uribesalgo
University: Universidad Autónoma de Madrid
Date: October 2005
Date: October 2005
Miguel Alcalde Laboratory
Institute of Catalysis, ICP-CSIC,
Cantoblanco, 28049, Madrid, Spain
Tel: +34 915854806
Fax: +34 915854760
