PhD Thesis
Directed evolution of ancestral and modern enzymes
Student: Bernardo Gomez Fernandez
University: Universidad Autónoma de Madrid
Date: 18/10/2019
Directed and computational evolution of fungal laccases: redox potential enhancement and development of a family of thermostable chimeras
Student: Ivan Mateljak
University: Universidad Autónoma de Madrid
Date:18/10/2017
Diseño de la peroxigenasa inespecífica de Agrocybe aegerita mediante evolución dirigida: expresión funcional en levaduras
Student: Patricia Molina Espeja
University: Universidad Autónoma de Madrid
Date: 31/3/2016
Biotechnology platforms for aryl-alcohol oxidases by directed evolution
Student: Javier Viña Gonzalez
University: Universidad Autónoma de Madrid
Date: 03/10/2019
Evolución dirigida de lacasas fúngicas: termoestabilidad y síntesis de colorantes heteropoliméricos
Student: Ana Isabel Vicente Martín
University: Universidad Complutense de Madrid
Date: 09/03/2017
Diseño de lacasas fúngicas activas en sangre mediante evolución molecular dirigida
Student: Diana Maté Mate
University: Universidad Autónoma de Madrid
Date: 25/06/2013
Evolución molecular dirigida de lacasas fúngicas en Saccharomyces cerevisiae: tolerancia a disolventes orgánicos
Student: Miren Zumárraga Uribesalgo
University: Universidad Autónoma de Madrid
Date: September 2007
Evolución dirigida de la peroxigenasa inespecífica de Agrocybe aegerita: Tolerancia a disolventes orgánicos mediante deriva genética neutral y evolución adaptativa
Student: Javier Martín Díaz
University: Universidad Autónoma de Madrid
Date: 24/07/2019
Evolución dirigida de la peroxidasa versátil para el diseño de una levadura de podredumbre blanca
Student: David González Pérez
University: Universidad Autónoma de Madrid
Date: 16/12/2016
Evolución Dirigida de peroxidasa versátil de Pleurotus eryngii en Saccharomyces cerevisiae
Student: Eva García Ruiz
University: Universidad Complutense de Madrid
Date: 15/11/2012
Síntesis enzimática de oligosacáridos de carácter prebiótico
Student: Arantza Gómez de Segura Ugalde
University: Universidad Autónoma de Madrid
Date: June 2004
Directed evolution of ancestral and modern enzymes
Student: Bernardo Gomez Fernandez
University: Universidad Autónoma de Madrid
Date: 18/10/2019
Biotechnology platforms for aryl-alcohol oxidases by directed evolution
Student: Javier Viña Gonzalez
University: Universidad Autónoma de Madrid
Date:03/10/2019
Evolución dirigida de la peroxigenasa inespecífica de Agrocybe aegerita: Tolerancia a disolventes orgánicos mediante deriva genética neutral y evolución adaptativa
Student: Javier Martín Díaz
University: Universidad Autónoma de Madrid
Date:24/07/2019
Directed and ccomputational evolution of fungal laccases: redox potential enhancement and development of a family of thermostable chimeras
Student: Ivan Mateljak
University: Universidad Autónoma de Madrid
Date:18/10/2017
Evolución dirigida de lacasas fúngicas: termoestabilidad y síntesis de colorantes heteropoliméricos
Student: Ana Isabel Vicente Martín
University: Universidad Complutense de Madrid
Date: 09/03/2017
Evolución dirigida de la peroxidasa versátil para el diseño de una levadura de podredumbre blanca
Student: David González Pérez
University: Universidad Autónoma de Madrid
Date: 16/12/2016
Diseño de la peroxigenasa inespecífica de Agrocybe aegerita mediante evolución dirigida: expresión funcional en levaduras
Student: Patricia Molina Espeja
University: Universidad Autónoma de Madrid
Date: 31/3/2016
Diseño de lacasas fúngicas activas en sangre mediante evolución molecular dirigida
Student: Diana Maté Mate
University: Universidad Autónoma de Madrid
Date: 25/06/2013
Evolución Dirigida de peroxidasa versátil de Pleurotus eryngii en Saccharomyces cerevisiae
Student: Eva García Ruiz
University: Universidad Complutense de Madrid
Date: 15/11/2012
Evolución molecular dirigida de lacasas fúngicas en Saccharomyces cerevisiae: tolerancia a disolventes orgánicos
Student: Miren Zumárraga Uribesalgo
University: Universidad Autónoma de Madrid
Date: September 2007
Síntesis enzimática de oligosacáridos de carácter prebiótico
Student: Arantza Gómez de Segura Ugalde
University: Universidad Autónoma de Madrid
Date: June 2004
Master Thesis
Evolución molecular dirigida de la aril-alcohol oxidasa de Pleurotus eryngii para la oxidación de alcoholes acrílicos secundarios
Student: Diego Esteban Lalana
University: Universidad Complutense de Madrid
Date: July 2018
University: Universidad Complutense de Madrid
Date: July 2018
Design of chimeric fusions of evolved Aryl alcohol oxidase-Unspecific peroxygenase in Saccharomyces cerevisiae
Student: Sofia Lazaro
University: Universidad Complutense de Madrid
Date: February 2018
University: Universidad Complutense de Madrid
Date: February 2018
Directed-focused-evolution of unspecific peroxygenase: improving thermostability and peroxygenase activity
Student: Miguel Arcángel Palomino
University: Universidad Autónoma de Madrid
Date: September 2016
University: Universidad Autónoma de Madrid
Date: September 2016
Directed evolution of aryl-alcohol oxidase using a tandem-yeast expression system
Student: Katarina Elbl
University: Faculty Applied Chemistry, Germany.
Date: January 2016
University: Faculty Applied Chemistry, Germany.
Date: January 2016
Chimeric signal peptides for functional expression of aryl alcohol oxidase in Saccharomyces cerevisiae
Student: Javier Viña González
University: Universidad Autónoma de Madrid
Date: July 2013
Engineering oxidative stability in versatile peroxidase by directed evolution
Student: Alina Ioana Roman
University: Universidad Autónoma de Madrid
Date: June 2011
Evolución molecular dirigida de la lacasa de alto potencial redox del hongo Pycnoporus cinnabarinus
Student: Patricia Molina Espeja
University: Universidad Autónoma de Madrid
Date: September 2009
Evolución molecular dirigida de lacasas de alto potencial redox
Student: Diana Maté Mate
University: Universidad Autónoma de Madrid
Date: June 2009
Unspecific peroxygenase engineering: analysis of structural and catalytic residues
Student: Maria Alejandra Alfuzzi
University: Universidad Autónoma de Madrid
Date: June 2018
University: Universidad Autónoma de Madrid
Date: June 2018
Directed evolution of a manganese peroxidase from Ceriporiopsis subvermispora towards functional expression
Student: Berndjan Eenink
University: Wageningen University, The Netherlands
Date: October 2016
University: Wageningen University, The Netherlands
Date: October 2016
Laboratory evolution of unspecific peroxygenase for activity in organic solvents
Student: Solymar J. del Pino
University: Universidad Autónoma de Madrid
Date: September 2016
University: Universidad Autónoma de Madrid
Date: September 2016
Shuffling the neutral drift of unspecific peroxygenase expressed in yeast
Student: Carmen Paret Fernández
University: Universidad Autónoma de Madrid
Date: July 2015
Engineering chimeric laccases by directed evolution
Student: Isabel Pardo
University: Universidad Autónoma de Madrid
Date: September 2011
Expresión funcional de peroxidasas de alto potencial redox mediante Evolución Dirigida
Student: Eva García Ruiz
University: Universidad Complutense de Madrid
Date: September 2009
Evolución dirigida de lacasas para su actividad en disolventes orgánicos
Student: Miren Zumárraga Uribesalgo
University: Universidad Autónoma de Madrid
Date: October 2005
Date: October 2005
Evolución molecular dirigida de la aril-alcohol oxidas de Pleurotus eryngii para la oxidación de alcoholes acrílicos secundarios
Student: Diego Esteban Lalana
University: Universidad Complutense de Madrid
Date: July 2018
University: Universidad Complutense de Madrid
Date: July 2018
Unspecific peroxygenase engineering: analysis of structural and catalytic residues
Student: Alejandra Alfuzzi
University: Universidad Autónoma de Madrid
Date: June 2018
University: Universidad Autónoma de Madrid
Date: June 2018
Design of chimeric fusions of evolved Aryl alcohol oxidase-Unspecific peroxygenase in Saccharomyces cerevisiae
Student: Sofia Lazaro
University: Universidad Complutense de Madrid
Date: February 2018
University: Universidad Complutense de Madrid
Date: February 2018
Directed evolution of a manganese peroxidase from Ceriporiopsis subvermispora towards functional expression
Student: Berndjan Eenink
University: Wageningen University, The Netherlands
Date: October 2016
University: Wageningen University, The Netherlands
Date: October 2016
Laboratory evolution of unspecific peroxygenase for activity in organic solvents
Student: Solymar J. del Pino
University: Universidad Autónoma de Madrid
Date: September 2016
University: Universidad Autónoma de Madrid
Date: September 2016
Directed-focused-evolution of unspecific peroxygenase: improving thermostability and peroxygenase activity
Student: Miguel Arcángel Palomino
University: Universidad Autónoma de Madrid
Date: September 2016
University: Universidad Autónoma de Madrid
Date: September 2016
Directed evolution of aryl-alcohol oxidase using a tandem-yeast expression system
Student: Katarina Elbl
University: Faculty Applied Chemistry, Germany.
Date: January 2016
University: Faculty Applied Chemistry, Germany.
Date: January 2016
Shuffling the neutral drift of unspecific peroxygenase expressed in yeast
Student: Carmen Paret Fernández
University: Universidad Autónoma de Madrid
Date: July 2015
Chimeric signal peptides for functional expression of aryl alcohol oxidase in Saccharomyces cerevisiae
Student: Javier Viña González
University: Universidad Autónoma de Madrid
Date: July 2013
Engineering chimeric laccases by directed evolution
Student: Isabel Pardo
University: Universidad Autónoma de Madrid
Date: September 2011
Engineering oxidative stability in versatile peroxidase by directed evolution
Student: Alina Ioana Roman
University: Universidad Autónoma de Madrid
Date: June 2011
Evolución molecular dirigida de la lacasa de alto potencial redox del hongo Pycnoporus cinnabarinus
Student: Patricia Molina Espeja
University: Universidad Autónoma de Madrid
Date: September 2009
Expresión funcional de peroxidasas de alto potencial redox mediante Evolución Dirigida
Student: Eva García Ruiz
University: Universidad Complutense de Madrid
Date: September 2009
Evolución molecular dirigida de lacasas de alto potencial redox
Student: Diana Maté Mate
University: Universidad Autónoma de Madrid
Date: June 2009
Evolución dirigida de lacasas para su actividad en disolventes orgánicos
Student: Miren Zumárraga Uribesalgo
University: Universidad Autónoma de Madrid
Date: October 2005
Date: October 2005
Miguel Alcalde Laboratory
Institute of Catalysis, ICP-CSIC,
Cantoblanco, 28049, Madrid, Spain
Tel: +34 915854806
Fax: +34 915854760
