DIRECTED ENZYME EVOLUTION GROUP AT ICP-CSIC
Welcome to Miguel Alcalde group at the Institute of Catalysis (ICP, CSIC, Madrid). Our research primarily focuses on the engineering of enzymes by directed evolution and computational design for a wide range of biotechnological purposes; the development of high-throughput biomolecular screening tools and genetic methods for library construction and exploration, as well as synthetic biology studies for environmental and energy applications.
DIRECTED EVOLUTION
Designing ad-hoc enzymes for industrial applications
1. Development of high-throughput screening assays
Highly sensitive and reliable screening assays to improve different biochemical traits.
2. Design of DNA recombination methods
Broad portfolio of library creation methods based on the high frequency of homologous recombination of Saccharomyces cerevisiae.
3. Analysis of mutant libraries assisted by computational algorithms.
Computational methods aimed at reducing screening efforts.
Recent highlighted publications
A repertoire of computationally designed peroxygenases for enantiodivergent C-H oxyfunctionalization reactions
Journal of the American Chemical Society 2023. In press.
Engineering a highly regioselective fungal peroxygenase for the synthesis of hydroxy fatty acids
Angewandte Chemie International Edition 2023, e202217372.
Surfing the wave of oxyfunctionalization chemistry by engineering fungal unspecific peroxygenases
Current Opinion in Structural Biology 2022. 73,102342.
Miguel Alcalde Laboratory
Institute of Catalysis, ICP-CSIC
Cantoblanco 28049, Madrid, Spain.
Tel: +34 915854806
Fax: +34 915854760
