DIRECTED ENZYME EVOLUTION GROUP AT ICP-CSIC
Welcome to Miguel Alcalde group at the Institute of Catalysis (ICP, CSIC, Madrid). Our research primarily focuses on the engineering of enzymes by directed evolution and computational design for a wide range of biotechnological purposes, the development of high-throughput biomolecular screening tools and genetic methods for library construction and exploration, as well as synthetic biology studies for environmental and energy applications.
Recent highlighted publications
Galactose oxidases engineered by directed evolution, atomistic design and ancestral sequence reconstruction
ACS Synthetic Biology 2025. 14: 239-246. https://doi.org/10.1021/acssynbio.4c00653
A repertoire of computationally designed peroxygenases for enantiodivergent C-H oxyfunctionalization reactions
Journal of the American Chemical Society 2023.145, 6, 3443–3453.
Directed evolution of LaccID for cell surface proximity labeling and electron microscopy
Nature Chemical Biology 2025 https://doi.org/10.1038/s41589-025-01973-6

Miguel Alcalde Laboratory






