DIRECTED ENZYME EVOLUTION GROUP AT ICP-CSIC
Welcome to Miguel Alcalde group at the Institute of Catalysis (ICP, CSIC, Madrid). Our research primarily focuses on the engineering of enzymes by directed evolution and computational design for a wide range of biotechnological purposes; the development of high-throughput biomolecular screening tools and genetic methods for library construction and exploration, as well as synthetic biology studies for environmental and energy applications.
DIRECTED EVOLUTION
Designing ad-hoc enzymes for industrial applications
1. Development of high-throughput screening assays
Highly sensitive and reliable screening assays to improve different biochemical traits.
2. Design of DNA recombination methods
Broad portfolio of library creation methods based on the high frequency of homologous recombination of Saccharomyces cerevisiae.
3. Analysis of mutant libraries assisted by computational algorithms.
Computational methods aimed at reducing screening efforts.
Recent highlighted publications
Ancestral resurrection and directed evolution of fungal Mesozoic laccases
Applied and Environmental Microbiology 2020, 86:e00778-20
The generation of thermostable fungal laccase chimeras by SCHEMA-RASPP structure-guided recombination in vivo
ACS Synthetic Biology 2019, 8: 833-843.
Increasing redox potential, redox mediator activity and stability in a fungal laccase by computer-guided mutagenesis and directed evolution
ACS Catalysis 2019, 9: 4561-4572.
Miguel Alcalde Laboratory
Institute of Catalysis, ICP-CSIC
Cantoblanco 28049, Madrid, Spain.
Tel: +34 915854806
Fax: +34 915854760
